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Your example of the kittens doesn't apply in real life (nature). Example: Nearly every Cocker Spaniel bred in the U.S. has their tail bobbed (and we've seen that for generations), but newborn Cocker Spaniels have full tails. Amputating an appendage doesn't change the genome of the parent, so offspring will have the genome to include the appendage that was amputated.
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Actualy this example was a true fact as it's what has been happening within the family(with generations of pussies) that is why I refered to it as it realy is amazing to witness.
As for genome change we do not have enough retreat to be able to affirm that no changes have occured, 30 or 40 years is far from being enough when it comes to seinf gene evolution.